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SiRNA Validation Data

siRNA ID:

s4648

Gene ID:

2011

Transcript(s):

NM_001039469.2 NM_001163296.1 NM_001163297.1 NM_004954.4 NM_017490.3 XM_011544789.1 XM_011544790.1 XM_011544791.1 XM_011544792.1 XM_011544793.1 XM_011544794.1 XM_011544795.1 XM_011544796.1 XM_011544797.1 XM_011544798.1 XM_017017310.1 XM_017017311.1

Validation Results:

18%(+/-5% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00245216_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5 nM gene-specific Silencer^® Select siRNA or 5 nM Silencer^® Select Negative Control #2 siRNA. 48 hours post-transfection, RNA was isolated, and then two-step, quantitative RT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.