SiRNA Validation Data

siRNA ID:

s14044

Gene ID:

7035

Transcript(s):

XM_005246818.1 XM_005246819.1 XM_006712720.3 XM_011511707.2 XM_011511708.1 XM_011511709.2

Validation Results:

2%(+/-1% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00196731_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5 nM gene-specific Silencer® Select siRNA or 5 nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, RNA was isolated, and then two-step, quantitative RT-PCR was performed using an appropriate TaqMan® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.