SiRNA Validation Data

siRNA ID:

s11634

Gene ID:

5832

Transcript(s):

NM_001017423.1 NM_001323412.1 NM_001323413.1 NM_001323414.1 NM_001323415.1 NM_001323416.1 NM_001323417.1 NM_001323418.1 NM_001323419.1 NM_002860.3

Validation Results:

4%(+/-1% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00161078_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.