SiRNA Validation Data

siRNA ID:

s29712

Gene ID:

Transcript(s):

NM_001289095.1 NM_001289096.1 NM_001289098.1 NM_001289099.1 NM_001289100.1 NM_001289101.1 NM_017771.4 XM_005265250.4 XM_005265252.4 XM_005265255.3 XM_005265256.3 XM_017006671.1 XM_017006672.1 XM_017006673.1 XM_017006674.1 XM_017006675.1 XM_017006676.1 XM_017006677.1 XM_017006678.1 XM_017006679.1 XM_017006680.1 XM_017006681.1 XM_017006682.1 XM_017006683.1 XM_017006684.1 XM_017006685.1 XM_017006686.1 XM_017006687.1 XM_017006688.1 XM_017006689.1 XM_017006690.1 XM_017006691.1 XM_017006692.1 XM_017006693.1 XM_017006694.1 XM_017006695.1 XM_017006696.1 XM_017006697.1 XM_017006698.1 XM_017006699.1 XM_017006700.1 XM_017006701.1 XM_017006702.1 XM_017006703.1 XM_017006704.1

Validation Results:

12%(+/-4% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00298958_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5 nM gene-specific Silencer^® Select siRNA or 5 nM Silencer^® Select Negative Control #2 siRNA. 48 hours post-transfection, RNA was isolated, and then two-step, quantitative RT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.