SiRNA Validation Data

siRNA ID:

s2984

Gene ID:

1108

Transcript(s):

NM_001273.3 NM_001297553.1 XM_005253668.3 XM_006718958.1 XM_006718959.1 XM_006718960.1 XM_006718961.2 XM_006718962.1 XM_017018725.1 XM_017018726.1 XM_017018727.1 XM_017018728.1 XM_017018729.1 XM_017018730.1 XM_017018731.1 XM_017018732.1 XM_017018733.1 XM_017018734.1

Validation Results:

10%(+/-1% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00172349_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5 nM gene-specific Silencer® Select siRNA or 5 nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, RNA was isolated, and then two-step, quantitative RT-PCR was performed using an appropriate TaqMan® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.