SiRNA Validation Data

siRNA ID:

135342

Gene ID:

Transcript(s):

NM_001024666.2 NM_031892.2 XM_005274494.2 XM_011545498.2 XM_011545499.2 XM_011545500.2 XM_011545503.2 XM_017029459.1 XM_017029460.1 XM_017029461.1 XM_017029462.1 XM_017029463.1 XM_017029464.1 XM_017029465.1 XM_017029466.1 XM_017029467.1 XM_017029468.1

Validation Results:

4%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00230414_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT NeoFX Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.