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SiRNA Validation Data

siRNA ID:

107187

Gene ID:

8481

Transcript(s):

NM_003611.2 XM_005274604.2 XM_005274606.3 XM_011545592.2 XM_011545594.2 XM_011545595.1 XM_011545597.1 XM_011545598.1 XM_017029909.1 XM_017029910.1 XM_017029911.1

Validation Results:

24%(+/-4% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00269273_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.