SiRNA Validation Data

siRNA ID:

121043

Gene ID:

Transcript(s):

NM_001174060.1 NM_017784.4 XM_005264843.3 XM_005264844.4 XM_005264845.1 XM_011533326.2 XM_017005667.1 XM_017005668.1 XM_017005669.1 XM_017005670.1

Validation Results:

4%(+/-1% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00215016_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT NeoFX Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.