SiRNA Validation Data

siRNA ID:

1692

Gene ID:

Transcript(s):

NM_001321309.1 NM_053025.3 NM_053026.3 NM_053027.3 NM_053028.3 XM_011512860.2 XM_011512861.2 XM_017006469.1 XM_017006470.1 XM_017006471.1 XM_017006472.1 XM_017006473.1

Validation Results:

12%(+/-5% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00178736_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT NeoFX Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.