Close X

SiRNA Validation Data

siRNA ID:

341

Gene ID:

5754

Transcript(s):

NM_001270398.1 NM_002821.4 NM_152880.3 NM_152881.3 NM_152882.3 NR_072997.1 NR_072998.1 XM_011514765.2 XM_011514766.2

Validation Results:

13%(+/-0% mRNA remaining)(HeLa cell line)

Validation Procedure:

The above cell line was plated in at least triplicate the day before the transfection procedure was started. siRNA was transfected at a final concentration of 100 nM. RNA was extracted from the cells 48 hours after transfection. mRNA levels were measured by real-time RT-PCR using a random primer mix for the RT priming, followed by real time PCR. Knockdown is expressed as the relative amount of target mRNA remaining compared to that of cells transfected with a "nonsense" or scrambled siRNA negative control. Validation results are shown for multiple independent experiments.