SiRNA Validation Data

siRNA ID:

105116

Gene ID:

Transcript(s):

NM_001128610.2 NM_001283049.1 NM_005154.4 XM_006720761.3 XM_006720762.3 XM_011522193.2 XM_017022718.1 XM_017022719.1 XM_017022720.1 XM_017022721.1 XM_017022722.1

Validation Results:

7%(+/-3% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00987109_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT NeoFX Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.