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SiRNA Validation Data

siRNA ID:

105270

Gene ID:

84749

Transcript(s):

NM_001301175.1 NM_032663.4 XM_005253962.3 XM_005253965.4 XM_006719653.3 XM_011538894.2 XM_017020048.1 XM_017020049.1 XM_017020050.1 XM_017020051.1 XM_017020052.1 XM_017020053.1 XM_017020054.1

Validation Results:

3%(+/-1% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00261902_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.