SiRNA Validation Data

siRNA ID:

1379

Gene ID:

65220

Transcript(s):

NM_001198993.1 NM_001198994.1 NM_001198995.1 NM_023018.4 XM_005244778.2 XM_006710837.2 XM_006710838.2 XM_006710839.1 XM_011542006.1 XM_011542007.1 XM_011542008.1 XM_011542009.2 XM_017002143.1 XM_017002144.1 XM_017002145.1 XM_017002146.1

Validation Results:

11%(+/-0% mRNA remaining)(HeLa cell line)

Validation Procedure:

The above cell line was plated in at least triplicate the day before the transfection procedure was started. siRNA was transfected at a final concentration of 100 nM. RNA was extracted from the cells 48 hours after transfection. mRNA levels were measured by real-time RT-PCR using a random primer mix for the RT priming, followed by real time PCR. Knockdown is expressed as the relative amount of target mRNA remaining compared to that of cells transfected with a "nonsense" or scrambled siRNA negative control. Validation results are shown for multiple independent experiments.