SiRNA Validation Data

siRNA ID:

413

Gene ID:

8394

Transcript(s):

NM_001135636.1 NM_001135637.1 NM_001135638.1 NM_003557.2 XM_005245525.4 XM_005245527.3 XM_005245528.4 XM_005245530.4 XM_005245531.3 XM_005245532.3 XM_006711563.3 XM_006711564.3 XM_006711567.3 XM_006711568.3 XM_011510043.2 XM_011510045.2 XM_011510046.2 XM_011510048.2 XM_011510049.2 XM_017002439.1 XM_017002440.1 XM_017002441.1

Validation Results:

13%(+/-0% mRNA remaining)(HeLa cell line)

Validation Procedure:

The above cell line was plated in at least triplicate the day before the transfection procedure was started. siRNA was transfected at a final concentration of 100 nM. RNA was extracted from the cells 48 hours after transfection. mRNA levels were measured by real-time RT-PCR using a random primer mix for the RT priming, followed by real time PCR. Knockdown is expressed as the relative amount of target mRNA remaining compared to that of cells transfected with a "nonsense" or scrambled siRNA negative control. Validation results are shown for multiple independent experiments.