SiRNA Validation Data

siRNA ID:

43735

Gene ID:

Transcript(s):

NM_001320586.1 NM_001320587.1 NM_001320588.1 NM_001320589.1 NM_001320590.1 NM_138448.3 XM_017005406.1 XM_017005407.1 XM_017005408.1 XM_017005409.1 XM_017005410.1 XM_017005411.1 XM_017005412.1 XM_017005413.1

Validation Results:

11%(+/-4% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00153848_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT NeoFX Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.