SiRNA Validation Data

siRNA ID:

999

Gene ID:

Transcript(s):

NM_016281.3 XM_005253897.1 XM_005253898.1 XM_006719445.2 XM_011538437.1 XM_011538439.1 XM_017019408.1 XM_017019409.1 XM_017019410.1 XM_017019411.1 XM_017019412.1 XM_017019413.1

Validation Results:

22%(+/-5% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00179599_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT NeoFX Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.