SiRNA Validation Data

siRNA ID:

s15930

Gene ID:

8394 266971

Transcript(s):

NM_001135636.1 NM_001135637.1 NM_001135638.1 NM_003557.2 XM_005245525.4 XM_005245527.3 XM_005245528.4 XM_005245530.4 XM_005245531.3 XM_005245532.3 XM_006711563.3 XM_006711564.3 XM_006711567.3 XM_006711568.3 XM_011510043.2 XM_011510045.2 XM_011510046.2 XM_011510048.2 XM_011510049.2 XM_017002439.1 XM_017002440.1 XM_017002441.1 NR_002319.2

Validation Results:

13%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00801004_s1]

Validation Procedure:

HeLa cells were reverse transfected with 5 nM gene-specific Silencer® Select siRNA or 5 nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, RNA was isolated, and then two-step, quantitative RT-PCR was performed using an appropriate TaqMan® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.