SiRNA Validation Data

siRNA ID:

s21184

Gene ID:

10783

Transcript(s):

NM_001145001.2 NM_001166167.1 NM_001166168.1 NM_001166169.1 NM_001166170.1 NM_001166171.1 NM_014397.5 XM_005251664.1 XM_006716936.2 XM_017014217.1

Validation Results:

17%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00205221_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.