SiRNA Validation Data

siRNA ID:

s22869

Gene ID:

23031

Transcript(s):

NM_015016.1 XM_005259823.3 XM_005259824.2 XM_005259825.2 XM_005259826.3 XM_005259827.2 XM_006722698.2 XM_006722699.2 XM_006722700.3 XM_011527823.1 XM_017026506.1 XM_017026507.1 XM_017026508.1 XM_017026509.1 XM_017026510.1 XM_017026511.1 XM_017026512.1 XM_017026513.1 XM_017026514.1 XM_017026515.1 XM_017026516.1

Validation Results:

11%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00390797_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5 nM gene-specific Silencer® Select siRNA or 5 nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, RNA was isolated, and then two-step, quantitative RT-PCR was performed using an appropriate TaqMan® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.