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SiRNA Validation Data

siRNA ID:

s25934

Gene ID:

27163

Transcript(s):

NM_001042402.1 NM_014435.3 XM_005262920.3 XM_005262921.3 XM_005262923.2 XM_005262924.2 XM_005262925.3 XM_006714180.2 XM_011531852.2 XM_011531854.2 XM_017008027.1 XM_017008028.1 XM_017008029.1 XM_017008030.1

Validation Results:

14%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00384501_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer^® Select siRNA or 5nM Silencer^® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan^® Gene Expression Cells-to-CT^™ Kit and appropriate TaqMan^® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.