SiRNA Validation Data

siRNA ID:

s35282

Gene ID:

65268

Transcript(s):

NM_001282394.1 NM_006648.3 XM_005252137.3 XM_005252140.2 XM_005252141.2 XM_005252144.2 XM_011518926.2 XM_011518927.2 XM_011518928.2 XM_011518930.2 XM_011518932.2 XM_011518937.2 XM_011518939.2 XM_011518940.2 XM_017015045.1 XM_017015046.1 XM_017015047.1 XM_017015048.1 XM_017015051.1

Validation Results:

11%(+/-4% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00198403_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.