SiRNA Validation Data

siRNA ID:

s35590

Gene ID:

79109

Transcript(s):

NM_001006617.1 NM_001006618.1 NM_001006619.1 NM_001006620.1 NM_001006621.1 NM_024117.3 XM_011519005.1 XM_011519006.2 XM_011519007.1 XM_011519008.1 XM_011519009.1 XM_017015126.1 XM_017015127.1

Validation Results:

3%(+/-1% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00225549_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.