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SiRNA Validation Data

siRNA ID:

1461

Gene ID:

3611

Transcript(s):

NM_001014794.2 NM_001014795.2 NM_001278441.1 NM_001278442.1 NM_004517.3 XM_005252904.4 XM_005252905.2 XM_011520065.1 XM_017017672.1

Validation Results:

11%(+/-0% mRNA remaining)(HeLa cell line)

Validation Procedure:

The above cell line was plated in at least triplicate the day before the transfection procedure was started. siRNA was transfected at a final concentration of 100 nM. RNA was extracted from the cells 48 hours after transfection. mRNA levels were measured by real-time RT-PCR using a random primer mix for the RT priming, followed by real time PCR. Knockdown is expressed as the relative amount of target mRNA remaining compared to that of cells transfected with a "nonsense" or scrambled siRNA negative control. Validation results are shown for multiple independent experiments.