SiRNA Validation Data

siRNA ID:

s32401

Gene ID:

56922

Transcript(s):

NM_001293273.1 NM_020166.4 NR_120639.1 NR_120640.1 XM_006713702.1 XM_011512992.2 XM_017006874.1 XM_017006875.1

Validation Results:

10%(+/-1% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00220127_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.