SiRNA Validation Data

siRNA ID:

103940

Gene ID:

Transcript(s):

NM_001297640.1 NM_001297642.1 NM_016546.3 XM_017019397.1 XM_017019398.1 XM_017019399.1 XM_017019400.1 XM_017019401.1 XM_017019402.1 XM_017019403.1 XM_017019404.1

Validation Results:

17%(+/-8% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00213057_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT NeoFX Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.