SiRNA Validation Data



Gene ID:



NM_001243027.2 NM_001243028.2 NM_001626.5 XM_011526614.1 XM_011526615.1 XM_011526616.1 XM_011526618.1 XM_011526619.1 XM_011526620.1 XM_011526622.2 XM_017026470.1 XM_017026471.1

Validation Results:

26%(+/-0% mRNA remaining)(HeLa cell line)

Validation Procedure:

The above cell line was plated in at least triplicate the day before the transfection procedure was started. siRNA was transfected at a final concentration of 100 nM. RNA was extracted from the cells 48 hours after transfection. mRNA levels were measured by real-time RT-PCR using a random primer mix for the RT priming, followed by real time PCR. Knockdown is expressed as the relative amount of target mRNA remaining compared to that of cells transfected with a "nonsense" or scrambled siRNA negative control. Validation results are shown for multiple independent experiments.