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SiRNA Validation Data

siRNA ID:

s1022

Gene ID:

109

Transcript(s):

NM_001320613.1 NM_004036.4 XM_006711925.1 XM_011532489.1 XM_011532490.1 XM_011532491.1 XM_011532492.2 XM_011532493.1 XM_011532494.1 XM_017003186.1 XM_017003187.1 XM_017003188.1 XM_017003189.1 XM_017003190.1

Validation Results:

16%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00269618_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer^® Select siRNA or 5nM Silencer^® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan^® Gene Expression Cells-to-CT^™ Kit and appropriate TaqMan^® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.