SiRNA Validation Data



Gene ID:



NM_000028.2 NM_000642.2 NM_000643.2 NM_000644.2 NM_000645.2 NM_000646.2 XM_005270557.2 XM_017000501.1

Validation Results:

19%(+/-3% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00240568_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.