SiRNA Validation Data



Gene ID:



NM_001123.3 NM_001202449.1 NM_006721.3 XM_017015698.1 XM_017015699.1 XM_017015700.1 XM_017015702.1 XM_017015703.1 XM_017015704.1 XM_017015706.1

Validation Results:

16%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00417073_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer® Negative Control #1 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.