Close X

SiRNA Validation Data

siRNA ID:

721

Gene ID:

10188

Transcript(s):

NM_001010938.1 NM_001308046.1 NM_005781.4 XM_005269268.3 XM_005269270.3 XM_005269274.3 XM_005269275.3 XM_011512317.2 XM_011512318.2 XM_011512320.1 XM_011512321.2 XM_017005508.1 XM_017005509.1 XM_017005510.1

Validation Results:

25%(+/-0% mRNA remaining)(HeLa cell line)

Validation Procedure:

The above cell line was plated in at least triplicate the day before the transfection procedure was started. siRNA was transfected at a final concentration of 100 nM. RNA was extracted from the cells 48 hours after transfection. mRNA levels were measured by real-time RT-PCR using a random primer mix for the RT priming, followed by real time PCR. Knockdown is expressed as the relative amount of target mRNA remaining compared to that of cells transfected with a "nonsense" or scrambled siRNA negative control. Validation results are shown for multiple independent experiments.