SiRNA Validation Data

siRNA ID:

s16238

Gene ID:

8536

Transcript(s):

NM_003656.4 XM_005265516.1 XM_005265517.2 XM_017007354.1

Validation Results:

5%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00269334_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5 nM gene-specific Silencer® Select siRNA or 5 nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, RNA was isolated, and then two-step, quantitative RT-PCR was performed using an appropriate TaqMan® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.