SiRNA Validation Data



Gene ID:



NM_001256834.2 NM_001284526.1 NM_001313950.1 NM_001313951.1 NM_001313952.1 NM_001313953.1 NM_001313954.1 NM_004217.3 NR_132730.1 XM_011524070.2 XM_011524072.2 XM_017025307.1 XM_017025308.1 XM_017025309.1 XM_017025310.1 XM_017025311.1

Validation Results:

13%(+/-5% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00177782_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5 nM gene-specific Silencer® Select siRNA or 5 nM Silencer® Select Negative Control #2 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step, quantitative RT-PCR was performed using an appropriate TaqMan® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.