SiRNA Validation Data

siRNA ID:

s2443

Gene ID:

847

Transcript(s):

NM_001752.3

Validation Results:

14%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00156308_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5 nM gene-specific Silencer® Select siRNA or 5 nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, RNA was isolated, and then two-step, quantitative RT-PCR was performed using an appropriate TaqMan® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.