SiRNA Validation Data

siRNA ID:

s25719

Gene ID:

27032

Transcript(s):

NM_001001485.2 NM_001001486.1 NM_001001487.1 NM_001199179.1 NM_001199180.1 NM_001199181.1 NM_001199182.1 NM_001199183.1 NM_001199184.1 NM_001199185.1 NM_014382.3 XM_005247354.2 XM_005247355.2 XM_005247356.2 XM_005247358.2 XM_017006164.1

Validation Results:

12%(+/-3% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00205122_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.