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SiRNA Validation Data

siRNA ID:

s12284

Gene ID:

6198

Transcript(s):

NM_001272042.1 NM_001272043.1 NM_001272044.1 NM_001272060.1 NM_003161.3 XM_011525101.2 XM_011525102.2 XM_011525103.2 XM_017024929.1 XM_017024930.1 XM_017024931.1 XM_017024932.1 XM_017024933.1

Validation Results:

5%(+/-1% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00177357_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5 nM gene-specific Silencer^® Select siRNA or 5 nM Silencer^® Select Negative Control #2 siRNA. 48 hours post-transfection, RNA was isolated, and then two-step, quantitative RT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.