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SiRNA Validation Data

siRNA ID:

s16

Gene ID:

649395 7531

Transcript(s):

NR_029404.1 NM_006761.4 NR_024058.1 XM_005256784.3 XM_017025005.1

Validation Results:

1%(+/-0% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00356749_g1]

Validation Procedure:

HeLa cells were reverse transfected with 5 nM gene-specific Silencer^® Select siRNA or 5 nM Silencer^® Select Negative Control #2 siRNA. 48 hours post-transfection, RNA was isolated, and then two-step, quantitative RT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.