SiRNA Validation Data

siRNA ID:

103563

Gene ID:

5213

Transcript(s):

NM_000289.5 NM_001166686.1 NM_001166687.1 NM_001166688.1 XM_005268974.1 XM_005268975.1 XM_005268976.3 XM_005268977.1 XM_005268978.2 XM_005268979.1 XM_011538487.1 XM_011538488.2 XM_017019467.1 XM_017019468.1 XM_017019469.1

Validation Results:

6%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00175997_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer® Negative Control #1 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.