SiRNA Validation Data

siRNA ID:

110566

Gene ID:

Transcript(s):

NM_000248.3 NM_001184967.1 NM_006722.2 NM_198158.2 NM_198159.2 NM_198177.2 NM_198178.2 XM_005264754.1 XM_005264755.3 XM_006713164.2 XM_011533722.2 XM_011533723.2 XM_011533725.1 XM_011533726.1 XM_017006444.1 XM_017006445.1 XM_017006446.1 XM_017006447.1 XM_017006448.1

Validation Results:

13%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00165156_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT NeoFX Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.