SiRNA Validation Data



Gene ID:



NM_001278549.1 NM_002610.4 NR_103729.1 NR_103731.1 XM_006712594.2 XM_006712595.3 XM_011511343.2 XM_011511344.2 XM_011511345.2 XM_011511347.2

Validation Results:

19%(+/-4% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00176853_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5 nM gene-specific Silencer® Select siRNA or 5 nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, RNA was isolated, and then two-step, quantitative RT-PCR was performed using an appropriate TaqMan® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.