SiRNA Validation Data



Gene ID:



NM_001168355.2 NM_001323504.1 NM_001323505.1 NM_001323506.1 NM_001323507.1 NM_003999.2 XM_005248384.1 XM_005248386.2 XM_005248387.2 XM_011514161.2 XM_017010019.1

Validation Results:

17%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00384278_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.