SiRNA Validation Data

siRNA ID:

s2427

Gene ID:

841

Transcript(s):

NM_001080124.1 NM_001080125.1 NM_001228.4 NM_033355.3 NM_033356.3 NM_033358.3 NR_111983.1 XM_005246885.1 XM_005246886.1 XM_005246887.1 XM_005246888.1 XM_005246889.1 XM_005246890.3 XM_005246891.4 XM_005246892.1 XM_005246893.2 XM_005246894.3 XM_005246895.2 XM_006712789.1 XM_006712790.3 XM_006712793.2 XM_011511969.1

Validation Results:

19%(+/-5% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00236278_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.