SiRNA Validation Data

siRNA ID:

s425

Gene ID:

55879

Transcript(s):

NM_018558.3 XM_011531184.2

Validation Results:

6%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00610921_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5 nM gene-specific Silencer® Select siRNA or 5 nM Silencer® Select Negative Control #2 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step, quantitative RT-PCR was performed using an appropriate TaqMan® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.