SiRNA Validation Data

siRNA ID:

118282

Gene ID:

Transcript(s):

NM_001287135.1 NM_001287136.1 NM_001287137.1 NM_012395.3 XM_005250436.3 XM_005250438.3 XM_005250439.2 XM_011516306.2 XM_011516307.2 XM_017012320.1 XM_017012321.1 XM_017012322.1

Validation Results:

18%(+/-9% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00202633_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT NeoFX Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.