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SiRNA Validation Data

siRNA ID:

1217

Gene ID:

1432

Transcript(s):

NM_001315.2 NM_139012.2 NM_139013.2 NM_139014.2 XM_006714998.3 XM_011514310.2 XM_017010299.1 XM_017010300.1 XM_017010301.1 XM_017010302.1 XM_017010303.1 XM_017010304.1

Validation Results:

28%(+/-0% mRNA remaining)(HeLa cell line)

Validation Procedure:

The above cell line was plated in at least triplicate the day before the transfection procedure was started. siRNA was transfected at a final concentration of 100 nM. RNA was extracted from the cells 48 hours after transfection. mRNA levels were measured by real-time RT-PCR using a random primer mix for the RT priming, followed by real time PCR. Knockdown is expressed as the relative amount of target mRNA remaining compared to that of cells transfected with a "nonsense" or scrambled siRNA negative control. Validation results are shown for multiple independent experiments.