SiRNA Validation Data

siRNA ID:

2327

Gene ID:

4258

Transcript(s):

NM_001204366.1 NM_001204367.1 NM_001204368.1 NM_002413.4 XM_006714221.2 XM_011531972.2 XM_017008211.1 XM_017008212.1 XM_017008213.1 XM_017008214.1

Validation Results:

6%(+/-1% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00182064_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer® Negative Control #1 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.