SiRNA Validation Data

siRNA ID:

35791

Gene ID:

Transcript(s):

NM_001135213.1 NM_001135214.1 NM_014776.3 NM_057169.3 NM_057170.3 NM_139201.2 XM_005253997.3 XM_005254000.3 XM_006719707.3 XM_006719708.3 XM_006719709.3 XM_006719712.3 XM_006719713.3 XM_006719714.3 XM_017020258.1 XM_017020259.1 XM_017020260.1 XM_017020261.1

Validation Results:

14%(+/-9% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00207498_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT NeoFX Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.