SiRNA Validation Data

siRNA ID:

39113

Gene ID:

Transcript(s):

NM_001304794.1 NM_001304795.1 NM_001304796.1 NM_152511.4 XM_005261368.4 XM_006724148.3 XM_011529920.2 XM_011529921.2 XM_017028627.1 XM_017028628.1

Validation Results:

15%(+/-8% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00380499_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT NeoFX Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.