SiRNA Validation Data

siRNA ID:

43929

Gene ID:

Transcript(s):

NM_001294338.1 NM_001294339.1 NM_003993.3 XM_005244878.1 XM_011509143.1 XM_011509144.1 XM_011509147.2 XM_017000241.1 XM_017000242.1 XM_017000243.1 XM_017000244.1

Validation Results:

22%(+/-4% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00241874_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT NeoFX Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.