SiRNA Validation Data

siRNA ID:

s14645

Gene ID:

7360

Transcript(s):

NM_001001521.1 NM_006759.3 XM_005264537.2 XM_005264538.1 XM_006712088.1 XM_011533091.1 XM_017004855.1 XM_017004856.1 XM_017004857.1

Validation Results:

4%(+/-1% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00198879_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5 nM gene-specific Silencer® Select siRNA or 5 nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, RNA was isolated, and then two-step, quantitative RT-PCR was performed using an appropriate TaqMan® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.