SiRNA Validation Data

siRNA ID:

s16640

Gene ID:

8732

Transcript(s):

NM_001286426.1 NM_001286428.1 NM_003800.4 XM_017011401.1 XM_017011402.1

Validation Results:

6%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00186930_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5 nM gene-specific Silencer® Select siRNA or 5 nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, RNA was isolated, and then two-step, quantitative RT-PCR was performed using an appropriate TaqMan® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.